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-   -   gel purification (http://www.molecularstation.com/forum/biology-forum/87259-gel-purification.html)

smitarao 01-01-2013 09:38 AM

gel purification
 
hiii frens
I am tryning to purify my desired amplicon (size 885 bp) from gel. I am using qiaquick gel extraction kit for this,but not getting good yeil and good 260/280 ratio when i analyse my sample on nanodrop after purification i want to use this sample for in vitro transcription.

ajithnandanam 03-28-2013 05:40 AM

Re: gel purification
 
Try re-eluting the column with half the volume of elution buffer.


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