Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum

Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum (http://www.molecularstation.com/forum/)
-   Biology Forum (http://www.molecularstation.com/forum/biology-forum/)
-   -   Animal-Derived Laboratory Reagents (http://www.molecularstation.com/forum/biology-forum/86957-animal-derived-laboratory-reagents.html)

fartbob5 12-06-2012 06:20 PM

Animal-Derived Laboratory Reagents
 
I have a question about animal-derived laboratory reagents and products. I’m working in a strictly in-vitro lab that focuses mainly on western blots and flow cytometry. I was wondering the cost to animal life associated with these reagents. These include:

FBS (fetal bovine serum) & BSA (bovine serum albumen): I figured these products are likely a byproduct of the slaughterhouse industry and therefore the animals are being slaughtered more for the meat than to get the blood to make these products. Am I right in thinking this?

Primary & secondary antibodies (raised in mouse, rabbit, goat, etc.): From what I understand there are three main types with differing degrees of cost to animal life
In vitro Monoclonal antibodies: Once the hybridoma is created they can be grown indefinitely in vitro so there is minimal cost to animal life. Am I right in thinking this?
In vivo (ascites) monoclonal antibodies: Does an animal have to die every time I place a new order for an antibody?
Polyclonal antibodies: Again, does an animal have to die every time I place a new order for an antibody?

Sheep’s blood agar: Unlike the bovine products, I wouldn’t think sheep are being killed for the meat so maybe they are being primarily slaughtered to generate these lab products. Am I right for thinking this?

I’m asking these questions because I enjoy working in the lab but just want to know the cost associated with the products I use in regards to animal life. Also, can you think of any other animal-derived reagants/products that I left out that could be used in in-vitro experiments?

Thanks for any help you can provide.


All times are GMT. The time now is 07:07 AM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved

Page generated in 0.09315 seconds with 11 queries