Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > General Science Forums > Biology Forum
Register Search Today's Posts Mark Forums Read

Biology Forum Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here!


Western Transfer

Western Transfer - Biology Forum

Western Transfer - Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here!


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 12-04-2012, 10:20 PM
Pipette Filler
Points: 66, Level: 1 Points: 66, Level: 1 Points: 66, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Dec 2012
Posts: 2
Thanks: 0
Thanked 0 Times in 0 Posts
Default Western Transfer



Hey guys,

I am running a western and I am a little confused during the transfer step.

When I have my gel (still between plates) in the transfer solution and as I am about to pry it open, should the gel be on the long plate (that way I can just easily slide it to filter paper) or short plate (will I need to flip the gel then). My PI said to have it on long plate that way I wouldn't get confused. But when I was prying it open it accidentally stuck to short plate and while I was trying to flip it over, the gel cracked into two pieces (luckily I was able to put the pieces back together). Sometimes I get the gel on long plate and sometimes it goes on short. How can I always ensure that this won't happen again. The orientation is really confusing me :/

Thanks,
Molecule2012
Reply With Quote
  #2  
Old 12-05-2012, 10:20 PM
Volunteer
Points: 200, Level: 3 Points: 200, Level: 3 Points: 200, Level: 3
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Dec 2012
Location: Morecambe
Posts: 34
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Western Transfer

Try doing it very slowly on the long plate yet if you see it doing otherwise just go with it. It won't change anything.
Reply With Quote
Reply

Tags
transfer , western


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
problem with transfer and western blotting of BN-PAGE maddy27 General Science Questions and Layperson Board 0 05-23-2012 07:51 AM
Transfer problems-smeared blot annie0202 Western Blot Forum 1 02-23-2011 04:10 PM
uneven transfer of western blot Jayakumar, R Protocols and Methods Forum 2 08-31-2003 04:27 AM
uneven transfer of western blot- solved but.... Jayakumar, R Protocols and Methods Forum 0 08-28-2003 02:42 PM
uneven transfer of western blot EK Protocols and Methods Forum 0 08-21-2003 05:36 PM


All times are GMT. The time now is 08:15 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11699 seconds with 16 queries