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#1
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| Hello, Is any one with solution to my problem? pls help! My PCR negative control is always giving a band on gel gel all the caution to prevent contamination has been observed including sterilisation of pipette and changing all the reagents ie mastermix, primer and water but still the problem persist. pls assist,. |
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#2
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| In organic chemistry, peptide synthesis is the production of peptides, which are organic compounds in which multiple amino acids are linked via amide bonds which are also known as peptide bonds. The biological process of producing long peptides (proteins) is known as protein biosynthesis. We use <a href="http://www.peptide2.com">peptide 2.0 Inc</a> for peptide synthesis. Synthesized peptides are used in applications such as designing enzymes, testing drugs, and creating antibodies. Rather than synthesizing their own peptides, many scientists outsource the job to custom services. There are two main avenues of peptide synthesis: solid-phase or liquid-phase synthesis. In the more common solid-phase synthesis, the C-terminus is protected by attachment to a solid resin, which also simplifies separating the peptide from the reaction mixture. Liquid-phase synthesis, or synthesis in solution, is slower and labor-intensive, but has the advantages of multiple rounds of purification, and the opportunity for convergent synthesis, in which synthesized peptides can be attached to form larger ones. Options available from most custom synthesis services include: design of the peptide with labels or modifications (such as phosphorylation, methylation, biotinylation, glycosylation, cyclization, or attachment to carrier proteins or dye labels); quantity; purification (most are purified by HPLC); verifiable purity (most are analyzed by mass spectrometry and/or analytical HPLC); and solubility testing. |
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| band , control , negative |
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| Thread | Thread Starter | Forum | Replies | Last Post |
| Always signal in negative control :( | pluto-nium244 | ChIP Chromatin Immunoprecipitation Forum | 3 | 11-17-2012 09:25 AM |
| Non-specific band in positive control but negative control not | Ruby_tran | PCR - Polymerase Chain Reaction Forum | 0 | 07-05-2011 07:31 AM |
| negative control gives band same as positive control | adiza | PCR - Polymerase Chain Reaction Forum | 4 | 04-26-2010 06:03 PM |
| negative control in Q-PCR how to analyze results? | gozde06 | Real-Time PCR and Quantitative PCR Forum | 0 | 02-27-2010 08:39 PM |
| Positive and Negative Control | agy lee | Microbiology Forum | 1 | 12-18-2003 06:39 PM |