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application of molecular biology in medicine,agriculture,forensic and other areas scientific endeavor
Last edited by khalyppo; 10-31-2012 at 06:09 AM.
Re: molecular biology
In organic chemistry, peptide synthesis is the production of peptides, which are organic compounds in which multiple amino acids are linked via amide bonds which are also known as peptide bonds. The biological process of producing long peptides (proteins) is known as protein biosynthesis. We use <a href="http://www.peptide2.com">peptide 2.0 Inc</a> for peptide synthesis. Synthesized peptides are used in applications such as designing enzymes, testing drugs, and creating antibodies. Rather than synthesizing their own peptides, many scientists outsource the job to custom services. There are two main avenues of peptide synthesis: solid-phase or liquid-phase synthesis. In the more common solid-phase synthesis, the C-terminus is protected by attachment to a solid resin, which also simplifies separating the peptide from the reaction mixture. Liquid-phase synthesis, or synthesis in solution, is slower and labor-intensive, but has the advantages of multiple rounds of purification, and the opportunity for convergent synthesis, in which synthesized peptides can be attached to form larger ones. Options available from most custom synthesis services include: design of the peptide with labels or modifications (such as phosphorylation, methylation, biotinylation, glycosylation, cyclization, or attachment to carrier proteins or dye labels); quantity; purification (most are purified by HPLC); verifiable purity (most are analyzed by mass spectrometry and/or analytical HPLC); and solubility testing.
|biology , molecular|
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