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pcr amplification

pcr amplification - Biology Forum

pcr amplification - Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here!


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Old 10-16-2012, 05:46 AM
Pipette Filler
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Question pcr amplification



i have already amplified and got product one month ago. But now while doing the same sample iam not getting in the same machine. WHAT CAN BE THE REASON FOR THAT?.
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Old 10-19-2012, 06:45 PM
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Default Re: pcr amplification

Sounds like it could be a sample issue. What temperature was the template stored it? Was it stored in sterile buffer? Are your Master Mix ingredients still good? Make sure your using the right concentrations of materials, as well as correct forward and reverse primers. Also try annealing step at 55 C.
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