Question on Enzymes
 

Although the Sodium Pump is an ATPase, during it's cycle there is a phosphate group being added and taken away from the protein so surely kinase and phosphotase are involved because an ATPase only hydrolyses ATP?

Also, we are just going over how Enzymes work with their substrates. Am I right in saying all enzymes work in the induced fit way where the enzyme is specific to it's substrate and involves the enzyme and the substrate working at an active site? For example DNA Ligase forms phosphodiester bonds between Okazaki fragments...

What about when one job involves two enzymes ie. DNA Gyrase and DNA Helicase in unwinding the double helix or when an enzyme has two jobs such as DNA Polymerase forming phosphodiester bonds between nucleotides and adding new nucleotides to the RNA Primer.

Re: Question on Enzymes
 

What an extra ordinary co incidence, that is exactly what I have studied couple of days before.
Thanks, many of the things are even more clearer to me from now on.

Re: Question on Enzymes
 

Bump
I helped? :lol:

Because I was looking for answers myself. Also, what exactly is Proteolytic Cleavage?

Re: Question on Enzymes
 

Induced fit is not always applicable

Re: Question on Enzymes
 

Proteolytic Cleavage is an enzyme activator.

Re: Question on Enzymes
 

Lactose operon induction principle (the central dogma) DNA RNA protein replication transcription and translation reverse transcription of RNA replication gene expression (gene expression) - gene transcription and translation process. Generating a variety of RNAs (tRNA, mRNA, rRNA) and various protein polypeptide chain. RNA (tRNA, mRNA, and rRNA gene expression according to the biological reaction of the internal and external environmental stimuli, gene expression in a manner divided into: constitutive expression induction and repression of expression of constitutive Gene expression at any stage of ontogeny can in most sustained gene expression in the cells, regardless of the expression level is high or low, it is less affected by environmental factors, or very little change, and the gene expression product is usually necessary, the life processes essential, such genes often referred to as housekeeping genes (housekeeping gene).  induction (induction): in the stimulation of specific environmental signal, the corresponding gene is activated, so that the gene expression product increase this type of gene known as inducible gene.  can induce gene expression in specific environments enhanced process called induction (induction).

Re: Question on Enzymes
 

In science, peptide synthesis is characterized as the formation of a peptide bond between two amino acids. While the definition of a peptide is not definitive, it usually refers to flexible (little secondary structure) chains of up to 30-50 amino acids. <a href="http://www.unitedpeptide.com">United Peptide</a> provide peptide synthesis services.

The ability to form peptide bonds to link amino acids together is over 100 years old, although the first peptides to be synthesized, including oxytocin and insulin, did not occur for another 50-60 years, demonstrating the difficult task of chemically synthesizing chains of amino acids (1). In the last 50 years, advances in protein synthesis chemistry and methods have developed to the point where peptide synthesis today is a common approach in even high-throughput biological research and product and drug development (2).

The benefit of peptide synthesis strategies today is that besides having the ability to make peptides that are found in biological specimens, creativity and imagination can be tapped to generate unique peptides to optimize a desired biological response or other result. This page highlights the important aspects of peptide synthesis, the most common methods of synthesis and purification and the strengths and limitations of the respective strategies.