I culture PBMCs (peripheral blood mononuclear cells) and I wonder if I have memory B cells in the culture. I know that there is almost no plasmacytes coz their per cent in full blood is very low. What about memory B cells?
If answer is "yes, there are memory B cells in your culture" then here goes another question. If I stimulate Th cells in culture only with anti-CD3 and thanks to their stimulation B cells are also stimulated so is it possible that memory B cells will response? Is anti-CD3 stimulation a secondary response for memory B cells? Anti-CD3 binds TCR on Th so is this sth new for them or normal situation coz they always catch sth through TCR? I think it is sth new coz they won't find sth so ideally similat to anti-CD3 in nature so even if I have memory B cells in culture they won't react on that.