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Molecular biology question!
Hello all, my name is boa and im an undergrad student in sociology in France. I have some science courses to take in my curriculumm and molecular biology is one of them. I am glad I found this forum and im sure it will be helpful to me throughout the semester!
Here is my question:
You have cloned a cDNA molecule in the EcoRI and PstI sites of pBR322. Which
bacterial strain (XLI-blue or JM105) are you going to use for the transformation
experiment and briefly explain why?
Xl1-blue : lac- F’ [ lacIq lacZ∆M15 Tn10(tetr)]
JM105 : lac- F’ [ proAB+ lacIqlacZ∆M15]
. I understand that since I added my foreign DNA at the amp resistant site, this gene will no longer be expressed...but im not sure how this helps me to solve the problem.
Re: Molecular biology question!
DiART ( <a href="http://www.protein2.com">www.protein2.com</a> ), Deuterium Isobaric Amine Reactive isobaric Tags which share the amine-reactive chemistry used by iTRAQ and TMT that synthesize amine-derivatized peptides from protein digests.
The innovative advantages:
1) DiART-labeled peptides are chromatographically identical.
2) Have much stronger low-mass reporter ions than that of iTRAQ abd TMT.
3) Low price, at least 50% lower than iTRAQ and TMT
|biology , molecular , question|
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