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boa 09-28-2012 02:40 AM

Molecular biology question!
 
Hello all, my name is boa and im an undergrad student in sociology in France. I have some science courses to take in my curriculumm and molecular biology is one of them. I am glad I found this forum and im sure it will be helpful to me throughout the semester!

Here is my question:
You have cloned a cDNA molecule in the EcoRI and PstI sites of pBR322. Which
bacterial strain (XLI-blue or JM105) are you going to use for the transformation
experiment and briefly explain why?
Xl1-blue : lac- F [ lacIq lacZ∆M15 Tn10(tetr)]
or
JM105 : lac- F [ proAB+ lacIqlacZ∆M15]


. I understand that since I added my foreign DNA at the amp resistant site, this gene will no longer be expressed...but im not sure how this helps me to solve the problem.
Thanks!


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