Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > General Science Forums > Biology Forum
Register Search Today's Posts Mark Forums Read

Biology Forum Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here!


Milk DNA extraction high 230,280nm

Milk DNA extraction high 230,280nm - Biology Forum

Milk DNA extraction high 230,280nm - Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here!


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 09-23-2012, 12:43 PM
Pipette Filler
Points: 191, Level: 3 Points: 191, Level: 3 Points: 191, Level: 3
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Aug 2012
Posts: 2
Thanks: 0
Thanked 0 Times in 0 Posts
Default Milk DNA extraction high 230,280nm



I am doing Cow milk DNA study, I use CTAB, pehol-chlorm extraction,freezing cool ethanol extraction pellet twice. air dry, and dissolve in TE, but UV reading
read UV230 and 280 is as high as UV260, any body has good suggestion??
Reply With Quote
  #2  
Old 10-21-2012, 11:01 AM
Pipette Filler
Points: 77, Level: 1 Points: 77, Level: 1 Points: 77, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Oct 2012
Posts: 5
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Milk DNA extraction high 230,280nm

awesome result. thanks buddy.
you are great.
Reply With Quote
  #3  
Old 10-22-2012, 06:01 PM
Kneeanderthal's Avatar
Pipette Filler
Points: 68, Level: 1 Points: 68, Level: 1 Points: 68, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Oct 2012
Posts: 26
Thanks: 0
Thanked 1 Time in 1 Post
Default Re: Milk DNA extraction high 230,280nm

Sounds like phenol contamination in your prep (don't like using phenol-chloro extractions for that reason). In this case running a gel with a standard would probably give you a better understanding of the quantity and quality of your DNA.
Reply With Quote
Reply

Tags
230 , 280nm , dna , extraction , high , milk


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
260/280 ratio very high in RNA extraction melanogaster General Science Questions and Layperson Board 0 03-09-2012 12:39 PM
Human Cytome Project - Update 24 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 1 08-01-2010 02:18 PM
Extraction of whole blood DNA and RNA bioteke DNA Extraction Forum 0 07-07-2010 03:33 AM
>>>> LOWER CHOLESTEROL <<<< Janis Stroud Forum Physik 0 09-22-2009 04:39 PM
Human Cytome Project - Update 6 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 0 01-06-2005 11:18 AM


All times are GMT. The time now is 05:15 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.15850 seconds with 16 queries