| | |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| Biology Forum Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here! |
| | LinkBack | Thread Tools | Display Modes |
|
#1
| |||||||||||
| |||||||||||
| I am doing Cow milk DNA study, I use CTAB, pehol-chlorm extraction,freezing cool ethanol extraction pellet twice. air dry, and dissolve in TE, but UV reading read UV230 and 280 is as high as UV260, any body has good suggestion?? |
|
#2
| |||||||||||
| |||||||||||
| awesome result. thanks buddy. you are great. |
|
#3
| ||||||||||||
| ||||||||||||
| Sounds like phenol contamination in your prep (don't like using phenol-chloro extractions for that reason). In this case running a gel with a standard would probably give you a better understanding of the quantity and quality of your DNA. |
| Tags |
| 230 , 280nm , dna , extraction , high , milk |
| Thread Tools | |
| Display Modes | |
|
|
| | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| 260/280 ratio very high in RNA extraction | melanogaster | General Science Questions and Layperson Board | 0 | 03-09-2012 11:39 AM |
| Human Cytome Project - Update 24 Jan. 2005 | Peter Van Osta | Cell Biology and Cell Culture | 1 | 08-01-2010 02:18 PM |
| Extraction of whole blood DNA and RNA | bioteke | DNA Extraction Forum | 0 | 07-07-2010 03:33 AM |
| >>>> LOWER CHOLESTEROL <<<< | Janis Stroud | Forum Physik | 0 | 09-22-2009 04:39 PM |
| Human Cytome Project - Update 6 Jan. 2005 | Peter Van Osta | Cell Biology and Cell Culture | 0 | 01-06-2005 10:18 AM |