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stripping of pvdf membrane with ponceau

stripping of pvdf membrane with ponceau - Biology Forum

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Old 08-01-2012, 09:30 AM
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Default stripping of pvdf membrane with ponceau



Hi friends,

How to do pvdf membrane stripping with ponceau stain? I heard its a very good method for stripping..................

plz help me....
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Old 08-21-2012, 01:28 PM
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Default Re: stripping of pvdf membrane with ponceau

Procedure to destain PVDF (polyvinylidene difluoride) membrane stained with Ponceau S : After proteins are transferred onto membrane, immerse the membrane in Ponceau S staining solution and stain for 5 minutes. After proteins have been visualized, rinse the membrane with distilled water and rapidly immerse in an aqueous solution of 0.1 M NaOH. Protein bands will start to disappear after 10-30 seconds. Rinse membrane with running distilled water for 2-3 minutes. Continue with other procedure being followed (e.g. immunological detection).
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