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#1
08-01-2012, 09:30 AM
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 stripping of pvdf membrane with ponceau Hi friends, How to do pvdf membrane stripping with ponceau stain? I heard its a very good method for stripping.................. plz help me....  |
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#2
08-21-2012, 01:28 PM
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| Re: stripping of pvdf membrane with ponceau Procedure to destain PVDF (polyvinylidene difluoride) membrane stained with Ponceau S : After proteins are transferred onto membrane, immerse the membrane in Ponceau S staining solution and stain for 5 minutes. After proteins have been visualized, rinse the membrane with distilled water and rapidly immerse in an aqueous solution of 0.1 M NaOH. Protein bands will start to disappear after 10-30 seconds. Rinse membrane with running distilled water for 2-3 minutes. Continue with other procedure being followed (e.g. immunological detection). |
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| Tags |
| membrane , ponceau , pvdf , stripping |
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