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STR typing for human tissue immersed in formalin solution

STR typing for human tissue immersed in formalin solution - Biology Forum

STR typing for human tissue immersed in formalin solution - Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here!


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Old 06-07-2012, 09:23 AM
Pipette Filler
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Default STR typing for human tissue immersed in formalin solution



Hello, this is Nancy ... i would like plz to ask about a DNA extraction protocol from a muscle tissue taken from an unkown dead body and immersed since 10 days in formalin (4C) (it was immersed by the autopsist and delivered like this to our lab) . i need to get a DNA profile to match it with the potentiel parents. i tried chelex, invitorgen for extraction (Identifiler, minifiler as PCR kit) .. i got no profile ... i need a protocol to get rid of formaldehyde and stg to break the crosslinks ... dunno ... i need to get a DNA profile some help plz
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