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-   -   STR typing for human tissue immersed in formalin solution (http://www.molecularstation.com/forum/biology-forum/85870-str-typing-human-tissue-immersed-formalin-solution.html)

nancy_nicolas 06-07-2012 09:23 AM

STR typing for human tissue immersed in formalin solution
 
Hello, this is Nancy ... i would like plz to ask about a DNA extraction protocol from a muscle tissue taken from an unkown dead body and immersed since 10 days in formalin (4C) (it was immersed by the autopsist and delivered like this to our lab) . i need to get a DNA profile to match it with the potentiel parents. i tried chelex, invitorgen for extraction (Identifiler, minifiler as PCR kit) .. i got no profile ... i need a protocol to get rid of formaldehyde and stg to break the crosslinks ... dunno ... i need to get a DNA profile :D some help plz


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