I have question regarding 5'RACE.I am not using kit to do RACE and it is my first time to do RACE. i tried so many times, but i got no results. i changed everything in the pcr conditions even i used gradient PCR. i think my primers is ok where it should have the G adapter to attach to the polyC tail tm around 68 and are 30 nucleotides.
i don't know what to do. is there is a problem in my samples?how do I know if the tdt enzyme was working or not?Do it have method to see whether the tdt enzyme was adding nucleotides to the cDNA.
can anyone help me, please