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I would like to ask an question. My qPCR has kind of unexpected results. At attachment is the file from BioRad iQ5. There you can see 5 samples in 5 different RNA concentrations (200, 100, 50, 25, 12,5 ng/ul). The cDNA was pre-amplified by pre-PCR (the B row is not). Can someone tell me his/her opinion about the first A row, please?
Thank you for your time.
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