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What is PCR ?

What is PCR ? - Biology Forum

What is PCR ? - Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here!


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  #1  
Old 05-16-2012, 02:24 PM
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Default What is PCR ?



What is PCR ?
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Old 05-20-2012, 08:39 AM
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Default Re: What is PCR ?

Thanks for the nice information.
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Old 05-23-2012, 07:32 AM
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Default Re: What is PCR ?

PCR (polymerase chain reaction) is a method to analyze a short sequence of DNA (or RNA) even in samples containing only minute quantities of DNA or RNA. PCR is used to reproduce (amplify) selected sections of DNA or RNA. Previously, amplification of DNA involved cloning the segments of interest into vectors for expression in bacteria, and took weeks. But now, with PCR done in test tubes, it takes only a few hours. PCR is highly efficient so that untold numbers of copies can be made of the DNA. Moreover, PCR uses the same molecules that nature uses for copying DNA:

Two "primers", short single-stranded DNA sequences that are synthesized to correspond to the beginning and ending of the DNA stretch to be copied;
An enzyme called polymerase that moves along the segment of DNA, reading its code and assembling a copy; and
A pile of DNA building blocks that the polymerase needs to make that copy.
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Old 05-24-2012, 01:41 PM
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Default Re: What is PCR ?

The polymerase chain reaction (PCR) is a technique for copying a piece of DNA a billion-fold. As the name suggests, the process creates a chain of many pieces, in this case the pieces are nucleotides and the chain is a strand of DNA. PCR is an enzyme-mediated reaction, and as with any enzyme, the reaction must occur at the enzyme's ideal operating temperature. The enzymes that are used for the PCR are DNA-dependent DNA polymerases (DDDP) derived from thermophilic (heat-loving) bacteria. As such, the enzymes function at higher tempertaures than the enzymes we commonly use in the laboratory or have working in our bodies.

Thanks a lot,
Calvin
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Old 07-28-2012, 11:40 AM
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Default Re: What is PCR ?

The polymerase chain reaction (PCR) is a biochemical technology in molecular biology to amplify a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence.

Thanks a lot,
John
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Old 08-16-2012, 12:39 PM
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Default Re: What is PCR ?

The polymerase chain reaction (PCR) is a biochemical technology in molecular biology to amplify a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence.
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Old 08-24-2012, 09:20 AM
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Default Re: What is PCR ?

Hi... guy's.
Thanks for nice information.
According to me PCR is a fast and inexpensive technique used to amplify, or make many copies of, small segments of DNA. This is necessary because methods used for analyzing DNA require more DNA than may be in a typical sample. A particularly useful feature of PCR is that it allows the amplification process to be limited to specifically targeted segments of the DNA mixture--such as the Y chromosome markers used in genealogical testing.

Nice to meet you.
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Old 09-08-2012, 06:55 PM
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Default Re: What is PCR ?

polymerase chain reaction
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Old 09-12-2012, 11:04 AM
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Default Re: What is PCR ?

PCR, or Polymerase Chain Reaction is defined as a molecular technique which allows the production of large quantities of a specific DNA from a DNA template using a simple enzymatic reaction without a living organism, such as E. coli or yeast.
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