| | Re: give the information about primer concentrations
10x or 10nM?
Let us look if it is 10nM (you can calculate yourself if other conc require by looking at example below)
M1 = 45nM (initial stock conc)
V1 = X, denote volume require of the stock
M2 = 10nM conc to archieve
V2 = 25uL, final volume to archieve
put it all in you get
X=5.5555, or 5.56uL
Thus in a 25uL reaction you require to pipette stock primer volume of 5.56uL.
**Important, when using M1V1=M2V2 make sure you SI unit is the same for volume and conc.