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problems with negative control in PCR

problems with negative control in PCR - Biology Forum

problems with negative control in PCR - Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here!


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Old 04-17-2012, 06:24 PM
Pipette Filler
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Default problems with negative control in PCR



Hi,

I am trying to amplify microsatellite loci from Olea europaea. I am using 0.5um of primer and I am getting a band of the expected size (between 200-300bp), yet I am getting roughly the same band in the negative control (no template DNA)! Could this be the result of primer dimers rather than amplified product? I am using about 200ng of DNA and performing a touch down PCR
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