Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > General Science Forums > Biology Forum
Register Search Today's Posts Mark Forums Read

Biology Forum Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here!


crazy about SDS- Page

crazy about SDS- Page - Biology Forum

crazy about SDS- Page - Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here!


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 04-17-2012, 06:00 AM
Pipette Filler
Points: 94, Level: 1 Points: 94, Level: 1 Points: 94, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Apr 2012
Posts: 2
Thanks: 0
Thanked 0 Times in 0 Posts
Default crazy about SDS- Page



Hi, i'm new to this group. I'm doing a research about lentil seed storage protein during germination. I face problem to run SDS-Page as I couldnt see any band even the standard maker after staining and destaining! May i know where is my problem? i'm totally blank out! @@

I just hope that anyone can guide me from top to the end about this SDS-Page. thanks....
Reply With Quote
  #2  
Old 04-17-2012, 08:16 PM
Pipette Filler
Points: 58, Level: 1 Points: 58, Level: 1 Points: 58, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Mar 2012
Posts: 2
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: crazy about SDS- Page

Have you already tried it with another SDS device?
When you use a prestained marker, does it remain in the pockets or can see you see it drifting through the gel? What about your protein samples? they simply remain where you pipetted them?
What are your electric current settings?
Do you see air bubbles after connecting the device to a power source?

You need to specificy your problem better.
Reply With Quote
  #3  
Old 05-04-2012, 01:54 PM
Pipette Filler
Points: 94, Level: 1 Points: 94, Level: 1 Points: 94, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Apr 2012
Posts: 2
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: crazy about SDS- Page

Thanks for give me reply...I think all of above i checked and finally i solve the problem already... the next problem is skewed band,i juz put 5 micronL in each pocket and set the current 30mA with 180V...howto improve it?
Reply With Quote
Reply

Tags
crazy , page , sds


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Page gel Gilbride, Kevin Protocols and Methods Forum 0 04-30-2008 02:15 PM
Page gel Jayakumar, R Protocols and Methods Forum 0 04-27-2008 07:55 PM
SDS PAGE younes sadramehr Protocols and Methods Forum 2 12-05-2006 07:21 PM
suggestions for new PAGE apparatus Jayakumar, R Protocols and Methods Forum 0 08-03-2005 09:53 PM
FS: Old Physics-Related Books Oldbooks78 Physics Forum 0 08-27-2003 02:32 PM


All times are GMT. The time now is 08:59 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.14074 seconds with 16 queries