TOP/FOP assay normalization
I am doing TOP/FOP assay for detection of regulation of wnt signalling. Problem arise when FOP is giving you higher reading in few cell lines. If there is someone like me and found the solution of this problem I will be thankful to him.
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Re: TOP/FOP assay normalization
facing same problem dude in mouse and human cell lines. these cells were not treated with any regulator except top/fop transfection.
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