I am trying to understand the importance of proliferation limits and cell senescence. In particular, how does the proliferation limit differ between Embryonic Stem cells (ES) and fibroblasts (which would be trans-differentiated). How does this difference plays a role when thinking about engineering organs, for example a heart transplant.
Any help? How do ES cells and fibroblasts compare with regards to the Hayflick limit?
Here is what I am thinking, however I am not sure if this is correct or not.
Non-differentiated the ES cells can divide almost indefinitely, however when the stem cell differentiates into a specific function, it goes through approximately 50 cell divisions before senescence. On the other hand, fibroblasts have already gone through some cell divisions at the point when they are trans-differentiated. Therefore, their life before senescence is less than 50 cell divisions.
Can someone verify this?