Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > General Science Forums > Biology Forum
Register Search Today's Posts Mark Forums Read

Biology Forum Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here!


Failed PCRs with a primer

Failed PCRs with a primer - Biology Forum

Failed PCRs with a primer - Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here!


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 03-01-2012, 11:38 AM
Pipette Filler
Points: 1,415, Level: 22 Points: 1,415, Level: 22 Points: 1,415, Level: 22
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Apr 2010
Posts: 3
Thanks: 0
Thanked 0 Times in 0 Posts
Default Failed PCRs with a primer



Hi All,
I am not getting good amplified products on trying a new primer of ropC gene in cyanobacteria. Surprisingly, i did not design the primer myself and had infact taken it from previous reports. Even the PCR conditions are same except the primer concentration which is 20uM (as in the paper) in comparison to the 0.5 uM that I use.
What should I do now...
Attached Images
File Type: jpg rpo C of 22nd feb 2012.jpg (6.4 KB, 2 views)
File Type: jpg rpo C gene 23rd feb 2012.jpg (6.1 KB, 2 views)
Reply With Quote
  #2  
Old 03-02-2012, 03:50 AM
Pipette Filler
Points: 1,415, Level: 22 Points: 1,415, Level: 22 Points: 1,415, Level: 22
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Apr 2010
Posts: 3
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Failed PCRs with a primer

is there anyone????
Reply With Quote
Reply

Tags
failed , pcrs , primer


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Urgent Help needed with this question. PCR theory ilovescience PCR - Polymerase Chain Reaction Forum 2 04-08-2011 05:05 PM
Second Guessing myself on primer concentrations nebulus PCR - Polymerase Chain Reaction Forum 1 05-01-2010 07:49 PM
Bad primer design ? How can I know ? surt Bioinformatics 4 10-07-2009 07:24 AM
I need immediate help!! How to PCR primer designing? :help: veniesh PCR - Polymerase Chain Reaction Forum 2 08-20-2009 04:00 PM
please help me in primer dimer saly Real-Time PCR and Quantitative PCR Forum 0 09-06-2008 02:18 PM


All times are GMT. The time now is 04:07 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11696 seconds with 17 queries