Hello, i'm new at agarose gel electrophoresis ... i'm wondering why i can't c a band or a smear of extracted genomic DNA
i did an 0.8% agarose TBE 1X solution , 0.5ug/ml ethidium bromid, 1x bromophenol blue, 100 volts.
i got a :
- great separation of bromophenol blue (3 colors)
- good bands of PCR products
- size marker bands
- but no genomic bands for the extracted DNA from a reference sample (saliva) i know it's large bands (20-50kb) ... but why i can't just detect its presence in the well or on the gel?