| |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| Biology Forum Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here! |
 |
| | LinkBack | Thread Tools | Display Modes |
|
#1
02-16-2012, 05:59 PM
| |||||||||||
| |||||||||||
 Sequential phosphorylation I´m working on a protein that becomes phosphorylated following DNA damage. From in vivo results using phosphorylation mutants we can say the phosphorylation is sequential at two sites. We identified the kinases acting on both sites using kinase inhibitors and Ipp. My question is, how is it possible that when I perfomed in vitro kinase assays using the kinase acting at the second phosphorylation residue I get a phosphorylated band although the first residue is not modificated (the phosphorylation is sequential as concluded by in vivo results). Thank for yout time.  |
|
| Tags |
| in vitro , phosphorylation , sequential |
| Thread Tools | |
| Display Modes | |
|
|
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| Retinoblastoma phosphorylation | ruslan | Biochemistry Forum | 0 | 01-20-2012 12:53 AM |
| Identify potential phosphorylation sites of the given peptide.? | bob mark | Peptide Forum | 1 | 12-13-2011 12:48 PM |
| Antarctic Phosphatase(NEB) for vector phosphorylation. | ukshas | Molecular Cloning Forum | 1 | 02-01-2009 07:06 AM |
| oxidative phosphorylation | kydiet | Biochemistry Forum | 0 | 12-08-2008 06:44 PM |
| help! sequential assignments with NMR triple resonance experiments | Jenn | Protein Forum | 0 | 09-22-2005 02:02 AM |
Linear Mode
