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#1
02-03-2012, 10:40 PM
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 kcat/Km and kon Hi, I need a bit of help with enzyme kinetics: What is the reason why kcat/Km cannot be greater than kon (association rate constant)? I need a molecular or a biochemical explanation. I found in a textbook that kcat/Km provides the lower limit for kon and that kcat/Km has an upper (higher) limit set by the diffusion rate 10^8 1/(M s); but that is not enough, I want to understand what is happening in the system and what is the meaning of the limit. Thank you, mfiguera  |
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