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Help! White interphase ONLY AFTER back-extracting phenol/chloroform phase

Help! White interphase ONLY AFTER back-extracting phenol/chloroform phase - Biology Forum

Help! White interphase ONLY AFTER back-extracting phenol/chloroform phase - Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here!


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Old 01-30-2012, 07:08 PM
Pipette Filler
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Default Help! White interphase ONLY AFTER back-extracting phenol/chloroform phase



When extracting DNA from a proteinase K digestion [~20 ug/ml EZ; 10 mM Tris pH 8.0; 5 mM EDTA; 0.5% SDS] by phenol chloroform (P:C:IA: 25:24:1) I don't observe any coloration in the interface initially. I vortex to mix and spin to form the phases. When I back-extract the organic phase w/ 0.5 vol H2O I then notice a pronounced white interphase. What could this be and why don't I see it at the first separation? Thanks much.
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Old 02-01-2012, 12:16 PM
Pipette Filler
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Default Re: Help! White interphase ONLY AFTER back-extracting phenol/chloroform phase

Both will be liquid. However they will evaporate quite fast in RT. Especially methylene chloride.
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backextracting , interphase , phase , phenol or chloroform , white


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