| |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| Biology Forum Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here! |
 |
| | LinkBack | Thread Tools | Display Modes |
|
#1
01-21-2012, 07:43 AM
| |||||||||||
| |||||||||||
 Protein Quantitation..... Hi, I'm working on observing a protein on a western blot, I have been unsuccessful. I have tried concentrating my sample using acetone precipitation. I'm going to try and concentrate my sample more. any suggestion? Also, I will be starting new cell cultures using complete media consisting of DMEM w/10% FBS. Is there anyway to help my cells produce more protein?  |
|
#2
02-06-2012, 03:20 AM
| |||||||||||
| |||||||||||
| Re: Protein Quantitation..... I guess if you can't even detect your protein via Western Blot, it must be in very very low concentration or it is even not there. When I grew cells on DMEM I measured the general concentration proteins in the cell lysate just per spectrophotometry at 600 nm (standardized by using BSA standard concentrations). For IP I used about 650 µg, for Western I always used about 30µg of the cell material and it worked perfectly fine. |
|
| Tags |
| protein , quantitation |
| Thread Tools | |
| Display Modes | |
|
|
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| Human Cytome Project - Update 24 Jan. 2005 | Peter Van Osta | Cell Biology and Cell Culture | 1 | 08-01-2010 02:18 PM |
| Human Cytome Project - Update 6 Jan. 2005 | Peter Van Osta | Cell Biology and Cell Culture | 0 | 01-06-2005 10:18 AM |
| New Saccharomyces Sequences 11/27/04 | Mike Cherry | Yeast Forum | 0 | 11-28-2004 11:39 PM |
| New Saccharomyces Sequences 05/19/04 | SGD Sequences | Yeast Forum | 0 | 05-23-2004 04:06 PM |
| New Saccharomyces Sequences 09/10/03 | SGD Sequences | Yeast Forum | 0 | 09-11-2003 01:33 AM |
Linear Mode
