Growing anaerobic Clostridium on TSA agar

[Coldcast]

Is it possible to enumerate Clostridium spp. using a pour plate method on TSA agar?

I feel like since they are a soil bacterium they can grow on TSA just not as well as they would in the gut. I guess it would be possible to grow them using the pour plate method since they are anaerobic bacterium that could collect at the bottom side of the agar where oxygen is very very low.

Thanks!

[jazzlynblu]

The bottom side of the agar does not make the environment anaerobic. What you need is an anaerobic jar to create an anaerobic environment. You can prepare the TSA plates to grow Clostridium and put the plates into an anaerobic jar to grow them.

Otherwise, you may use cooked meat medium or fluid thioglycolate medium to grow them. (Both are broth.)

[Jorge1907]

Suggest you use surface plating with Oxyrase.