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Growing anaerobic Clostridium on TSA agar

Growing anaerobic Clostridium on TSA agar - Biology Forum

Growing anaerobic Clostridium on TSA agar - Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here!


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Old 01-20-2012, 01:40 AM
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Default Growing anaerobic Clostridium on TSA agar



Is it possible to enumerate Clostridium spp. using a pour plate method on TSA agar?

I feel like since they are a soil bacterium they can grow on TSA just not as well as they would in the gut. I guess it would be possible to grow them using the pour plate method since they are anaerobic bacterium that could collect at the bottom side of the agar where oxygen is very very low.

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Old 01-20-2012, 02:18 AM
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Default Re: Growing anaerobic Clostridium on TSA agar

The bottom side of the agar does not make the environment anaerobic. What you need is an anaerobic jar to create an anaerobic environment. You can prepare the TSA plates to grow Clostridium and put the plates into an anaerobic jar to grow them.

Otherwise, you may use cooked meat medium or fluid thioglycolate medium to grow them. (Both are broth.)
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Old 01-22-2012, 05:04 PM
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Default Re: Growing anaerobic Clostridium on TSA agar

Suggest you use surface plating with Oxyrase.
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