Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > General Science Forums > Biology Forum
Register Search Today's Posts Mark Forums Read

Biology Forum Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here!


real time pcr

real time pcr - Biology Forum

real time pcr - Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here!


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 01-11-2012, 04:56 AM
Pipette Filler
Points: 64, Level: 1 Points: 64, Level: 1 Points: 64, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jan 2012
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Question real time pcr



is this wise to do melting curve analysis when using taqman probe chemistry?
Reply With Quote
  #2  
Old 01-28-2012, 11:36 AM
Pipette Filler
Points: 85, Level: 1 Points: 85, Level: 1 Points: 85, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jan 2012
Posts: 4
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: real time pcr

Taqman is the trade mark. and taqman chemistry is hydrolysis. after the elongation probe was hydrolysied by polymerase which has 5' exonuclease activity. because of that there is nor any material to bind to amplicon. you dont do melting analysis when you use taqman chemistry.
Reply With Quote
Reply

Tags
pcr , real , time


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
the absolutely final complete collection of ideas blochee Physics Forum 2 06-15-2007 06:31 AM
The Achilles Heel of String Theory. S D Rodrian Physics Forum 7 07-08-2006 02:40 PM
Does any of this make sense ??? Yrael Physics Forum 3 09-12-2005 03:11 AM
Relativists have never answered this question Robert Calvert Physics Forum 84 12-17-2003 08:11 PM
The Law of Moving Dimenstions: Time Dimension is Moving Relative To Spatial Dimension Ranger West Physics Forum 6 11-12-2003 01:34 AM


All times are GMT. The time now is 01:38 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.14799 seconds with 16 queries