Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > General Science Forums > Biology Forum
Register Search Today's Posts Mark Forums Read

Biology Forum Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here!


Viscous DNA in DNA extraction

Viscous DNA in DNA extraction - Biology Forum

Viscous DNA in DNA extraction - Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here!


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 01-10-2012, 04:12 PM
Pipette Filler
Points: 22, Level: 1 Points: 22, Level: 1 Points: 22, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jan 2012
Posts: 4
Thanks: 0
Thanked 0 Times in 0 Posts
Default Viscous DNA in DNA extraction



Hello.

Im presently carryng out a molecular study with some plant species of the Family Lauracea (Persea mostly).

Presently, the DNA extraction is being carried out using Quiagen DNA extraction kit, with which I have had good results in the past with other plant species. Nevertheless, with several species of the Persea genus things are quite different.

I grind the plant material with liquid nitrogen and soon after I add the AP1 buffer (lyses buffer), a very viscous, mucus like substance results. If I carry on the protocol as recomended by Quiagen and add the AP2 buffer (precipitation buffer), centrifuge and after which I atempt to recover the supernatant, in several cases there is no supernatant to recovery. Just a block of a mucus in the tube.

Whenever any supernatant is recovered, it is translucent, although quite viscous. Carriyng out the extraction untill the end, in most cases there is no DNA obtained from the extraction, as evidenced by the absence of bands in a electrophoresis.

I have tried altering the procedure recomended by Quiagen in an atempt to obtain DNA, but untill now with little or no success. The alterations are mostly related to alterations in the volume of AP1 and/or AP2 used during the extraction.

Does anyone have similar experience in situations similar to this one and can you please give me some guidance on how to obtain DNA from plants that produce very viscous substances?

PS: I have tried extracting DNA from these species using CTAB. The very viscous substance also forms and in general the sucess of the DNA extraction in very low.

Thank you in advance for your possible interest and help in solving this issue.

Best wishes

Carlos Lobo
Reply With Quote
Reply

Tags
dna , extraction , viscous


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Plant DNA extraction problem calobo Protocols and Methods Forum 0 01-10-2012 04:03 PM
Problems in DNA extraction using kit calobo Molecular Biology Techniques 0 01-10-2012 03:59 PM
Extraction of whole blood DNA and RNA bioteke DNA Extraction Forum 0 07-07-2010 03:33 AM
DNA Extraction from viscous medium Dirk Mller Protocols and Methods Forum 2 03-01-2005 11:56 AM
DNA Extraction from viscous medium taskan4 Protocols and Methods Forum 0 03-01-2005 11:31 AM


All times are GMT. The time now is 09:39 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11546 seconds with 16 queries