I am using 2x sample buffer for running my lysates in SDS page. when i do so , I get a broad blue region below the BFB line (i.e below the sharp bromophenol bue indicator line). Further my BFB line is kind of thick instead of sharp.
Could any one face the same problem?? or any idea to overcome it
p.s my samples becomes very diluted when i go with 1x Sample buffer. also i hear the pH of resolving buffer and Sample buffer has some direct correlation.
recipe of SB is correct.
Help me out if you got any idea