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Question about yeast morphology

Question about yeast morphology - Biology Forum

Question about yeast morphology - Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here!


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  #1  
Old 09-27-2011, 05:04 PM
Pipette Filler
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Default Question about yeast morphology



Hello

For people that work with yeast as an organism model: I am expressing a human protein in yeast, with a Gal promoter, that appears to induce some kind of abnormalities in the yeast cell cycle, althought it does not cause yeast growth delay: at phase contrast, some cells appear elongated (more cylindric really) and some appear to form chains, like if the cytokinesis was not complete. Does anyone knows any biochemical method by which I can monitor this phenotype? I am currently making countings which is very subjective, despite being blinded experiments.
Can some one share some insights on this?
Thanks
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Old 10-11-2011, 09:31 AM
Ph.D Doctorate
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Default Re: Question about yeast morphology

Perhaps your affecting chitin synthesis or chitinase activity.
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Old 10-12-2011, 01:05 PM
Pipette Filler
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Default Re: Question about yeast morphology

Thank's fro the reply. I will check it out!
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