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Question about yeast morphology
For people that work with yeast as an organism model: I am expressing a human protein in yeast, with a Gal promoter, that appears to induce some kind of abnormalities in the yeast cell cycle, althought it does not cause yeast growth delay: at phase contrast, some cells appear elongated (more cylindric really) and some appear to form chains, like if the cytokinesis was not complete. Does anyone knows any biochemical method by which I can monitor this phenotype? I am currently making countings which is very subjective, despite being blinded experiments.
Can some one share some insights on this?
Re: Question about yeast morphology
Perhaps your affecting chitin synthesis or chitinase activity.
|morphology , question , yeast|
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