I wondered, if anyone used U6 as a reference gene not only for miRNA normalisation, but for the mRNA too?
I am using Qiagen primer assays, therefore the amplicons are similar size for the snRNA i.e. U6, miRNA and mRNA. Their technical support, adviced me that in principle- why not, that U6 can be used for mRNA normalisation. I am using their protocol where I extract Total RNA, including miRNA, and can use this sample for both: miRNA and mRNA detection. U6 looks all right in my treated and untreated samples, and I used it for my miRNA normalisation, so now it would be very convenient to use it for the mRNA analyses too. I wondered if somebody had a similar experience?