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#1
09-09-2011, 04:05 PM
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 RNU6B as a housekeeping gene for mRNA Hello, I wondered, if anyone used U6 as a reference gene not only for miRNA normalisation, but for the mRNA too? I am using Qiagen primer assays, therefore the amplicons are similar size for the snRNA i.e. U6, miRNA and mRNA. Their technical support, adviced me that in principle- why not, that U6 can be used for mRNA normalisation. I am using their protocol where I extract Total RNA, including miRNA, and can use this sample for both: miRNA and mRNA detection. U6 looks all right in my treated and untreated samples, and I used it for my miRNA normalisation, so now it would be very convenient to use it for the mRNA analyses too. I wondered if somebody had a similar experience? Thank you   |
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#2
09-14-2011, 06:47 AM
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 Re: RNU6B as a housekeeping gene for mRNA what about U6... i want to change to u6 too... |
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| gene , housekeeping , mrna , rnu6b |
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