This is a question from some study examples I have for a midterm coming up.
The sequence of bases 3’- …TCTGATA'T'CAGTACG … -5’ occurs along one chain of a DNA duplex that has opened up at a replication fork. If synthesis of an RNA primer on this template begins by copying the 'T' base, then what is the sequence of the first six nucleotides of this RNA primer?
Now, I figure that the RNA primer will consist bases complementary to those in the sequence above, but I'm not sure in what direction the primer bonds...
i.e., will it be the bases complementary to TGATA'T' or 'T'CAGTA?
I'm thinking it's the former of the two, because I think the RNA primer binds to the TATA box, and the complementary strand of the first six nucleotides would be ACTATA, but I'm not sure so I'm looking for some guidance