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RNA primer example during DNA replication.

RNA primer example during DNA replication. - Biology Forum

RNA primer example during DNA replication. - Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here!


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Old 03-17-2011, 03:52 AM
Pipette Filler
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Default RNA primer example during DNA replication.



This is a question from some study examples I have for a midterm coming up.

The sequence of bases 3’- …TCTGATA'T'CAGTACG … -5’ occurs along one chain of a DNA duplex that has opened up at a replication fork. If synthesis of an RNA primer on this template begins by copying the 'T' base, then what is the sequence of the first six nucleotides of this RNA primer?

Now, I figure that the RNA primer will consist bases complementary to those in the sequence above, but I'm not sure in what direction the primer bonds...

i.e., will it be the bases complementary to TGATA'T' or 'T'CAGTA?

I'm thinking it's the former of the two, because I think the RNA primer binds to the TATA box, and the complementary strand of the first six nucleotides would be ACTATA, but I'm not sure so I'm looking for some guidance

Thanks!
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Old 03-17-2011, 05:41 PM
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Default Re: RNA primer example during DNA replication.

The direction will be 5'->3'. Since the sequence you put above is 3'->5', the RNA primer would be synthetized to the right. In other words, it would be complementary to the
3'-'T'CAGTA-5' sequence. The RNA primer sequence would then be: 5'-'A'GUCAU-3'.

5'- A G U C A U -3'
3'- T C A G T A -5'

Why U, because it is a RNA primer.

Hope It helps!
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