Originally Posted by jones23
Hello I am new here. I am wondering if any of you guys use ORF Finder? from NCBI?
Just general knowledge here, why do 6 frames show up? what is the purpose of the 6 frames?
If you read DNA in one direction there are three possible "reading frames" you can have. For example, the following sequence
Can be read (as codons):
ATG CCT TAA
The ORF finder also looks at the possibility your sequence is backwards (i.e. your sequence is of the complementary, not coding, strand), giving you three additional reading frames.