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electroblotting of DNA ...doesit work with big fragments?

electroblotting of DNA ...doesit work with big fragments? - Biology Forum

electroblotting of DNA ...doesit work with big fragments? - Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here!


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Old 06-13-2010, 04:28 PM
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Default electroblotting of DNA ...doesit work with big fragments?



Hi All,Is it possible to blot large DNA fragments using electro blotting?
I used a 6% native PAGE with depurination.
My fragment size is ~19kb.
Do you think size does matter?!
I used the biorad apparatus.
It didnt work of course.
If the DNA can move down,
why cant it move across?
Any thoughts?

Last edited by Uday; 06-13-2010 at 04:32 PM. Reason: Spelling mistake
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Old 06-22-2010, 07:32 AM
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Default Re: electroblotting of DNA ...doesit work with big fragments?

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