Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > General Science Forums > Biology Forum
Register Search Today's Posts Mark Forums Read

Biology Forum Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here!


cleaning glasswares

cleaning glasswares - Biology Forum

cleaning glasswares - Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here!


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 05-15-2010, 06:48 AM
Pipette Filler
Points: 55, Level: 1 Points: 55, Level: 1 Points: 55, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: May 2010
Posts: 3
Thanks: 0
Thanked 0 Times in 0 Posts
Question cleaning glasswares



Hi to all friends
Can you inform me about the cleaning procedure for microbial lab glasswares?
Reply With Quote
  #2  
Old 05-17-2010, 11:23 PM
Phototoxin's Avatar
Pipette Filler
Points: 81, Level: 1 Points: 81, Level: 1 Points: 81, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: May 2010
Location: England
Posts: 8
Thanks: 0
Thanked 3 Times in 3 Posts
Default Re: cleaning glasswares

I dont know about 'microbial' labs ... but generally I used to use deionised/MQ water and then acetone to get rid of the water. It depends what materials you're cleaning out of what containers.
Reply With Quote
  #3  
Old 09-11-2010, 10:44 AM
Pipette Filler
Points: 777, Level: 15 Points: 777, Level: 15 Points: 777, Level: 15
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Dec 2009
Posts: 10
Thanks: 0
Thanked 1 Time in 1 Post
Default Re: cleaning glasswares

Generally you should first disinfect your glassware, using something like virkon (or just 70% Ethanol for some kind of microbiology). This will ensure that you're not going to wash your microbes into the drains and outside world.

Then you need to actually clean the glassware. Use a lab detergent for this, such as Decon 90 (which is what I use). Don't be tempted to use a household detergent, since they put various additives in these to leave your crockery all shiny, but it's not so good to contaminate your samples with these Depending on what you're doing, your glassware might need cleaning with something like isopropanol, ethanol or acetone to get rid of organics (but the detergent should do a pretty good job of this anyway).

Following cleaning, you need to get rid of the stuff that you used to clean the glassware, I do this with copious amounts of DI water. You really can't rinse enough at this stage. Then, finally rinse with 18 MΩ water (from a MilliQ machine for example) to get rid of any minerals that were dissolved in the DI water.

After rinsing, put the glassware directly into a drying oven to remove the water. You don't need to worry about drying the glassware with acetone if you use 18 MΩ water for a final rinse, since there should be nothing to leave "water marks" on the glassware in this water.

Finally, you can autoclave the glassware before reuse to kill off any bacteria that have grown on the glassware between cleaning and reuse.

This will leave you with very clean glassware, but you can reduce any of the stages if you don't need it super clean. Cleaner is generally better though, especially for the repeatability of experiments.
Reply With Quote
  #4  
Old 09-27-2010, 08:39 AM
Pipette Filler
Points: 75, Level: 1 Points: 75, Level: 1 Points: 75, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Sep 2010
Posts: 15
Thanks: 0
Thanked 1 Time in 1 Post
Default Re: cleaning glasswares

Thanks for the information ,I too needed to know it
Reply With Quote
  #5  
Old 02-16-2013, 09:13 AM
Pipette Filler
Points: 253, Level: 5 Points: 253, Level: 5 Points: 253, Level: 5
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Nov 2012
Posts: 7
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: cleaning glasswares

If the lab glassware is cleaned immediately after use then the problems associated with dirt can be controlled to a great extent. If detergent is used for laboratory glassware washing, it should be the one that is specially designed for lab glassware cleaning and much more knowldge about the lab glassware then visit to my site labsglassware
Reply With Quote
  #6  
Old 03-02-2013, 09:00 AM
Pipette Filler
Points: 25, Level: 1 Points: 25, Level: 1 Points: 25, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Feb 2013
Posts: 6
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: cleaning glasswares

What equipments do you have that you can use? It depends on your resources.
Reply With Quote
Reply

Tags
cleaning , glasswares


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
cleaning microbial glasswares niroomandi Molecular Biology Techniques 2 05-16-2010 10:08 AM
~ SERVICE APARTMENT ~ Abernathy Racanelli Botany Forum 0 07-16-2009 09:37 PM
tank cleaning (Padnos.Beth@epamail.epa.gov) (Jocelyn) Heather S McAllister (hmcallis@Princeton.EDU) Zebrafish Forum 0 09-04-2008 05:43 PM
tank cleaning (Padnos.Beth@epamail.epa.gov) Heather S McAllister (hmcallis@Princeton.EDU) Zebrafish Forum 1 09-03-2008 06:02 PM
cleaning basin, bathtub and shower walls w/o using standard cleaning agents sold at the stores. amanda Chemistry Forum 4 07-19-2003 03:15 AM


All times are GMT. The time now is 02:57 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.14271 seconds with 16 queries