Alright, so I'm trying to learn the methodology and theory behind Western Blot, but I have this question that's been nagging me, and I can't seem to find a specific answer to it.
Why is gel electrophoresis necessary in the Western Blot process?
I know how it separates proteins based on their Molecular Weight, but what is the point when you've got an antibody tag that's going to bind to a specific protein. I mean, my reasoning is that if that antibody interacts with the homogenate of a particular cell line, then isn't that a basis for gene expression and the presence of a specific protein. Somebody please help.