Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > General Science Forums > Biology Forum
Register Search Today's Posts Mark Forums Read

Biology Forum Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here!


Fluorescence assays and absorbance assays

Fluorescence assays and absorbance assays - Biology Forum

Fluorescence assays and absorbance assays - Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here!


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 12-08-2009, 08:26 PM
Pipette Filler
Points: 67, Level: 1 Points: 67, Level: 1 Points: 67, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Dec 2009
Posts: 3
Thanks: 2
Thanked 0 Times in 0 Posts
Default Fluorescence assays and absorbance assays



What is the difference between fluorescence and absorbance assays?
Reply With Quote
  #2  
Old 12-08-2009, 09:47 PM
danfive's Avatar
Nobel Laureate
Points: 8,612, Level: 64 Points: 8,612, Level: 64 Points: 8,612, Level: 64
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jul 2007
Location: Houston TX
Posts: 969
Thanks: 12
Thanked 184 Times in 154 Posts
Default Re: Fluorescence assays and absorbance assays

Well the answer is in the definition.

fluorescence assay--will emit light, thus you will measure the emitted light as the signal of the assay.

absorbance assay--will produce a color, the intensity of the color will absorb a given wavelength (say 450nm) and that is the measured signal.
Reply With Quote
The Following User Says Thank You to danfive For This Useful Post:
HTS76 (12-09-2009)
Reply

Tags
absorbance , assays , fluorescence


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
protein and carbohydrate assays. analysing absorbance and concentration RachBio ELISA Assay Forum 0 02-07-2009 12:29 AM


All times are GMT. The time now is 01:47 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11356 seconds with 16 queries