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10-18-2009, 03:38 AM
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 how do you know if a frame shift is real or by sequencing error? Hi guys, I am new here. I have a question regarding a bioinformatics assignment. We assembled ESTs from wheat into contigs, and then blasted those contigs on blastx, to look for genes in the caleosin family. One of the contigs had a frame shift. There was a question in the assignment asking that if there is a frame shift present in the sequence, how do you know if it is real or by sequencing error or error in sequence assembly? So how do I figure that out?  |
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