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Blue/white screening?

Blue/white screening? - Biology Forum

Blue/white screening? - Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here!


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Old 06-22-2009, 12:16 PM
Pipette Filler
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Default Blue/white screening?



amplicons(gene of interest) are ligated into plasmid vector..thus i assume the plasmid's ability to produce b-galactosidase is disrupted..but this plasmid-amplicon thing is transformed into e.coli cell and grown on LB agar..

so whether the colony is blue/or white is because the plasmid lac z becomes disrupted OR the e.coli lac z becomes disrupted?

my guess is the the e.coli used is treated and cannot produce b-galactosidase in the first place
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