Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum

Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum (http://www.molecularstation.com/forum/)
-   Biology Forum (http://www.molecularstation.com/forum/biology-forum/)
-   -   Blue/white screening? (http://www.molecularstation.com/forum/biology-forum/67190-blue-white-screening.html)

Zeke 06-22-2009 12:16 PM

Blue/white screening?
 
amplicons(gene of interest) are ligated into plasmid vector..thus i assume the plasmid's ability to produce b-galactosidase is disrupted..but this plasmid-amplicon thing is transformed into e.coli cell and grown on LB agar..

so whether the colony is blue/or white is because the plasmid lac z becomes disrupted OR the e.coli lac z becomes disrupted?

my guess is the the e.coli used is treated and cannot produce b-galactosidase in the first place


All times are GMT. The time now is 03:37 AM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved

Page generated in 0.08817 seconds with 11 queries