amplicons(gene of interest) are ligated into plasmid vector..thus i assume the plasmid's ability to produce b-galactosidase is disrupted..but this plasmid-amplicon thing is transformed into e.coli cell and grown on LB agar..
so whether the colony is blue/or white is because the plasmid lac z becomes disrupted OR the e.coli lac z becomes disrupted?
my guess is the the e.coli used is treated and cannot produce b-galactosidase in the first place
|All times are GMT. The time now is 01:00 AM.|
Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved