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VEGF from Abcam 46154 IHC-PLEASE HELP

VEGF from Abcam 46154 IHC-PLEASE HELP - Biology Forum

VEGF from Abcam 46154 IHC-PLEASE HELP - Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here!


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Old 04-08-2009, 08:21 AM
Pipette Filler
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Default VEGF from Abcam 46154 IHC-PLEASE HELP



Hi all,
I am new around here and desperate for help from you all So I joined this forum.
I am really fighting with this antibody VEGF from Abcam 46154. I does not work for me acording to the web instructions in parafin sections. I tried all the tricks. Has anybody worked with it successfully?
I have very good experience with Abcam antibodies so what is going on ?
thank you very much in advance,
summer
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Old 04-09-2009, 04:34 PM
Pipette Filler
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Default Re: VEGF from Abcam 46154 IHC-PLEASE HELP

Hi Summer,
We have had at least one report of this working well on human and mouse paraffin-embedded tissue using a heat-mediated antigen retrieval with citrate buffer, pH 6, followed by blocking steps and the antibody at 1/100.
Please note that the secondary was a polymer goat anti-rabbit IgG, which amplifies the signal more effectively than a standard conjugated secondary.
The full review can be at the review link on the datasheet for ab46154.
If you have already tried a similar protocol, please contact us at [Only registered users see links. ] and we'll see what we can do to help.
Abcam
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