I am using the BrDU from Roche (cat# 11296736001; it is for DNA synthesis) to detect the DNA breaks and I got some problems regarding this techniques:
(1) According to a paper: From Chromatin-bound PCNA complex formation triggered by DNA damage occurs independent of the ATM gene product in human cells. I can use the BrDU to detect the DNA breaks, I want to know why this kit can detect the DNA breaks? What is the mechanism?
(2) When I use BrDU to tell the cell that exits from cell cycle, I got almost no BrDU positive staining, does that mean for the cell that exits from cell cycle, there is no DNA synthesis? And if there is no DNA synthesis, do you think I can get DNA repair if I break the DNAs by using cobalt radiation or Bleomycin, please?