Originally Posted by hershee
many many thanks danfive,
your reply is absolutly valuable..
actually i wanted my stock of 0.016M to be diluted in 10nano molar concentration in final volume of 5ml.....another solution from stock is 20 nano molar in 5ml final vol.
then i will have 2-3 nano molar conc in 5ml..
is it easy?
#1--you want 5ml of 10nM solution
setup as: 16,000nM x V1 = 10nM x 5mL
solve for V1 = (10nM x 5mL)/16,000nM = 0.003125mL= 3.125uL
Means you should mix 3.125 microliters of 0.016M cisplatin into 5mL saline
#2--you want 5ml of 20nM solution
setup as: 16,000nM x V1 = 20nM x 5mL
solve for V1 = (20nM x 5mL)/16,000nM = 0.00625mL= 6.25uL
Means you should mix 6.25 microliters of 0.016M cisplatin into 5mL saline
Note: I didn't bother tracking the significant figures.