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The Biuret Method...

The Biuret Method... - Biology Forum

The Biuret Method... - Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here!


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Old 02-18-2009, 06:06 PM
Pipette Filler
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Default The Biuret Method...



Hi there,

Can anyone please help unconfuse me...

I have been searching the internet in an attempt to find a better understanding of the Biuret Method, but as of yet all that I have managed to do is confuse myself even more...

I beleive its... To determine the protein concentration of unknown solutions from known protein concentrations using a spectrophotometer...

But this is rather basic... can anyone explain it any better?
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Old 05-15-2009, 07:03 AM
Pipette Filler
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Default Re: The Biuret Method...

1.2. Método de Biuret
Se basa en la formación de un complejo coloreado entre el Cu2+ y los grupos NH de los enlaces peptídicos en medio básico. 1Cu2+ se acompleja con 4 NH. La intensidad de coloración es directamente proporcional a la cantidad de proteínas (enlaces peptídicos) y la reacción es bastante específica, de manera que pocas sustancias interfieren. La sensibilidad del método es muy baja y sólo se recomienda para la cuantificación de proteínas en preparados muy concentrados (por ejemplo en suero).




its in spanish..im sorry.you can translate it and you will understand better...i hope!

I wish you success!

Jorge Amaya S.
Student Ing. in Biotecnology
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Old 05-15-2009, 07:04 AM
Pipette Filler
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Default Re: The Biuret Method...

1.2. Método de Biuret
Se basa en la formación de un complejo coloreado entre el Cu2+ y los grupos NH de los enlaces peptídicos en medio básico. 1Cu2+ se acompleja con 4 NH. La intensidad de coloración es directamente proporcional a la cantidad de proteínas (enlaces peptídicos) y la reacción es bastante específica, de manera que pocas sustancias interfieren. La sensibilidad del método es muy baja y sólo se recomienda para la cuantificación de proteínas en preparados muy concentrados (por ejemplo en suero).




its in spanish..im sorry.you can translate it and you will understand better...i hope!

I wish you success!

Jorge Amaya S.
Student Ing. in Biotecnology
Reply With Quote
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