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Ventricular Endothelial Cell Isolation

Ventricular Endothelial Cell Isolation - Biology Forum

Ventricular Endothelial Cell Isolation - Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here!


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Old 01-16-2009, 03:21 AM
Pipette Filler
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Default Ventricular Endothelial Cell Isolation



I currently isolate ventricular cardiomyocyes form neonatal rat pups using a percol gradient. In the gradient we get a clear band of myocytes, another nbnad of no-myocyte cells and cell debris and red blood cells at the bottom. Has anyone ever isolated endothelial cells from the heart? Does anyone know a good cell surface marker and/or antibody that would be useful?

Any suggestions woudl be appreaciated.

-AB
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