I have isolated 9 soil bacteria and sent for sequencing. I had identified them using BLASTn. The results showed that 5 of them belong to genus Bacillus, 2 strains belong to genus Serratia , 1 strain is genus Pseudomonas and 1 strain belong to genus Escherichia.
Now, I would like to construct phylogenetic tree. However, I not sure how to do it.
So, can I just construct a phylogenetic tree that only consists 9 strains of my study bacteria samples? Or
do I need to construct a phylogenetic tree that consists of my study isolated bacteria together with some related bacteria species??
Please help me to remove all my doubt... Thanks.