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juannajuann 05-29-2013 06:10 AM

blastn and phylogenetic tree
 
Hi,

I just received the sequencing lists for my unknown bacterial. There are 5 unknown bacteria that I need to identify.
I would like to identify the bacteria using blastn and construct phylogenetic tree using neighbor joining.
Can anyone help me how to do it, since I not sure and familiar with it. Please help me as fast as possible.
Thanks.

Below are the lists of my sequencing results.


>1st_BASE_1215970_B_subtilis_168_Bacillus_Family_F .ab1
GGATTTCATATATTCGTCCTACAGCTAGCGTCATTGGAACTGGCATGTAA ACTGGGATACCTCCGGGAAACCGGGGCTGA
TACCGGATGGTGGTTTAAACCGCATGGTTCAAACATAAAAGGTGGCTTCG GCTACCACTTACAGATGGACCCGCGGCGCA
TTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGA CCTGAGAGGGTGATCGGCCACACTGGGACT
GAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCA ATGGACGAAAGTCTGACGGAGCAACGCCGC
GTGAGTGATGAAGGTTTTCGGATCGTAAAGCTCTGTTGTTAGGGAAGAAC AAGTACCGTTCGAATAGGGCGGTACCTTGA
CGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTA ATACGTAGGTGGCAAGCGTTGTCCGGAATT
ATTGGGCGTAAAGGGCTCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCC CCCGGCTCAACCGGGGAGGGTCATTGGAAA
CTGGATA

>1st_BASE_1212247_TKM_2_Bacillus_Family_F.ab1
TGGAAGGGGGGTATTGGGAACTGGAGATTGCCGTGGGTAACTTGCCTGTA AGACTGGGATAACTCCGGGAAACCGGGGCT
AATACCGGATGCTTGTTTGAACCGCATGGTTCAAACATAAAAGGTGGCTT CGGCTACCACTTACAGATGGACCCGCGGCG
CATTAGTTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCC GACCTGAGAGGGTGATCGGCCACACTGGGA
CTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCG CAATGGACGAAAGTCTGACGGAGCAACGCC
GCGTGAGTGATGAAGGTTTTCGGATCGTAAAGCTCTGTTGTTAGGGAAGA ACAAGTACCGTTCGAATAGGGCGGTACCTT
GACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGG TAATACGTAGGTGGCAAGCGTTGTCCGGAA
TTATTGGGCGTAAAGGGCTCGCAGGCGGTTTCTTAAGTCTGATGTGAAAG CCCCCGGCTCAACCGGGGAGGGTCATTGGA
AACTGGATAA

>1st_BASE_1212249_TKM_6_Bacillus_Family_F.ab1
NGGGGGAACGGAGTAGGGGGGACGGAGAGTACACGTGGGTAACCTGCCTG TAAGACTGGGATAACTCCGGGAAACCGGGG
CTAATACCGGATGCTTGTTTGAACCGCATGGTTCAAACATAAAAGGTGGC TTCGGCTACCACTTACAGATGGACCCGCGG
CGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAG CCGACCTGAGAGGGTGATCGGCCACACTGG
GACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTC CGCAATGGACGAAAGTCTGACGGAGCAACG
CCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAGCTCTGTTGTTAGGGAA GAACAAGTACCGTTCGAATAGGGCGGTACC
TTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGC GGTAATACGTAGGTGGCAAGCGTTGTCCGG
AATTATTGGGCGTAAAGGGCTCGCAGGCGGTTTCTTAAGTCTGATGTGAA AGCCCCCGGCTCAACCGGGGAGGGTCATTG
GAAACTGGAAAA

>1st_BASE_1214998_NL_3A_Bacillus_Family_F.ab1
TTTGGGGGGTGGGAGGGGGAACTGGAGCTAGCGCGTGGGTAACCTGCCTG TAAGACTGGGATACCTCCGGGAAACCGGGG
CTAATACCGGATGCTTGTTTGAACCGCATGGTTCAAACATAAAAGGTGGC TTCGGCTACCACTTACAGATGGACCCGCGG
CGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAG CCGACCTGAGAGGGTGATCGGCCACACTGG
GACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTC CGCAATGGACGAAAGTCTGACGGAGCAACG
CCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAGCTCTGTTGTTAGGGAA GAACAAGTACCGTTCGAATAGGGCGGTACC
TTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGC GGTAATACGTAGGTGGCAAGCGTTGTCCGG
AATTATTGGGCGTAAAGGGCTCGCATGCGGTTTCTTAAGTCTGATGTGAA AGCCCCCGGCTCAACCGGGGAGGGTCATTG
GAAACTGGATAA

>1st_BASE_1215001_CNL_2A_16S_532F2.ab1
NNNNNNNTNNNGACGTGAGCGNTACGCGNCNTGCTGACACANNCGTTTCT TGTGTNGATGCCCCCCCCGCAATNCCCTGG
GAGCTGCATCCAAAACTGGCGAGCTAGAGTACGGTAGAGGGTGGTGGAAT TTCCTGTGTAGCGGTGAAATGCGTAGATAT
AGGAAGGAACACCAGTGGCGAAGGCGACCACCTGGACTGATACTGACACT GAGGTGCGAAAGCGTGGGGAGCAAACAGGA
TTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCAACTAGCCGTTGGA ATCCTTGAGATTTTAGTGGCGCAGCTAACG
CATTAAGTTGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTCAAATG AATTGACGGGGGCCCGCACAAGCGGTGGAG
CATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACAAN


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